Less sensitive human epithelial tumor cell lines.Įffect of scorpion venom treatment on p53, bax and bcl-2 genes expression in Hela and A549 cells.Ī. Most sensitive human epithelial tumor cell lines.ī. The percentage of cell proliferation was measured by MTT cytotoxicity assay.Ī. The cells were incubated for 72hr with scorpion venom at concentrations of 0.1-1mgml. Graphics of cytotoxicity of scorpion venom against different cell lines. In most sensitive tumor cells (A549), scorpion venom induced necrosis evidenced by acridine orangeethidium bromide fluorescent dyes and down-expression of apoptosis-related genes. Scorpion venom demonstrated to induce apoptosis in less sensitive tumor cells (Hela) as evidenced by chromatin condensation, over expression of p53 and bax mRNA, down expression of bcl-2 mRNA and increase of activated caspases 3, 8, 9. There was no effect on either normal or hematopoietic tumor cells. Only the epithelial cancer cells showed significant cell viability reduction, with medium cytotoxic concentration (IC50) ranging from 0.6-1mgml, in a concentration-dependent manner. However, there is little scientific evidence about its potential in cancer therapy.Īdditionally, the effect of venom on tumor cell death was assayed by Fluorescence microscopy, RT-PCR and western blot. There w as no effect on either normal or hematopoietic tumor cells.
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